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KMID : 0364820090450020208
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Korean Journal of Microbiology
2009 Volume.45 No. 2 p.208 ~ p.214
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Reevaluation of Enumeration of Bacillus cereus Grown on Mannitol-Egg York-Polymyxin B Agar
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Yun Suk-Hyun
Kim Yong-Sang
Jeong Do-Youn
Hahn Kum-Su
Uhm Tai-Boong
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Abstract
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To avoid ambiguity in counting the number of colony, about 1,500 of colonies grown on B. cereus selective agar plates were grouped into 12 types by morphological difference and then identified by biochemical and 16S rDNA nucleotide sequence. Among them, seven colony types with 11 to 15 mm diameters of halo were identified as B. cereus or B. cereus subsp. cytotoxis. Five mm sized colonies with no halo, which have not been considered as B. cereus according to the manufacturer’s manual, were identified as B. cereus. A colony type with double halos of only 6 mm in diameter was also B. cereus. Other three types were proven to be Enterococcus sp., Brevibacillus sp., and B. subtilis, respectively. PCR results showed that only 9 types that are identified as B. cereus strains harbor at least one of B. cereus toxin genes.
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KEYWORD
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Bacillus cereus, Bacillus cereus selective media, microbial enumeration, microbial identification
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